Curriculum vitae
| Name: | Puchner |
| Vorname: | Elias |
| Geburtsdatum: | 18.01.1981 |
| Geburtsort: | Nürnberg |
| Adresse: | Horemansstr. 23, 80636 München |
Beruflicher Kontakt
Ludwig-Maximilians-Universität München
Lehrstuhl für Angewandte Physik (LS Gaub)
Amalienstr. 54
80799 München
Tel. 089-2180-2005
Bildung
- Seit 2008 Postdoc an der LMU München, Lehrstuhl für Angewandte Physik (LS Gaub)
- 2008 Promotion an der LMU München, Lehrstuhl für Angewandte Physik (LS Gaub)
"Einzelmolekül-Mechanoenzymatik" mit "summa cum laude" - 2006 Diplom in Physik/Biophysik an der LMU München, Lehrstuhl für Angewandte Physik (LS Gaub)
"Enzymmechanik – Einzelmolekülexperimente mit einem AFM-TIRF Hybrid". Note 1.0 - 2000-2006 Studium der Physik mit Schwerpunkt Biophysik und Nebenfach Biochemie an der LMU München
- 1998 "Summer-Term" an der Harvard University, Cambridge, MA, USA, "Fundamentals of Contemporary Astronomy"
- 1988-2000 Rudolf-Steiner Schule Nürnberg, Abitur
Praktika
- 4/04 – 6/04 Forschungsaufenthalt an der Columbia University, New York, USA, Dept. of Biological Sciences bei Prof. Julio Fernandez: "Construction of a combined magnetic tweezers -TIRF-hybrid and its application to protein unfolding"
- 3/04 - 4/04 Biochemisches Praktikum bei Prof. Beyer am Lehrstuhl für Stoffwechselbiochemie, LMU München: "Untersuchung von Lipidmembranen mit NMR"
- 2003 Werkstudent bei Dr. Wolfgang Parak, LMU München, Lehrstuhl für Angewandte Physik "Synthesis of highly fluorescing CdSe/ZnS nanocrystals"
Eingeladene Vorträge und Konferenzbeiträge
- 2009 59th meeting of the Nobel Laureates in Lindau. Talk: "Force and Function".
- 2009 Biophysical Society 53rd Annual Meeting, Boston, USA. Poster: "Single-Molecule Force Spectroscopy Reveals the Function of Titin Kinase as Force Sensor"
- 2008 X. Linz Winter Workshop "Advances in Single-Molecule Research for Biology & Nanoscience. Poster: "Comparing Proteins by their Unfolding Pattern"
Euro AFM Forum, Münster. Eingeladener Vortrag: "Single-Molecule Studies on the Mechanical Activation of the Molecular Force Sensor Titin Kinase" - 2007 European Biophysics Congress, London. Poster: "Single-Molecule Studies on the Mechanical Activation of the Molecular Force Sensor Titin Kinase"
- 2003 Nanoparticle Symposium, IPHT Jena, Poster: "Synthesis of highly fluorescing CdSe/ZnS nanocrystals"
Preise/Stipendien
- 2009 Publication prize CENS (Center for Nanoscience, Munich)
- 2008 Attocube Wittenstein Research Award
- 2008 Publikationspreis CENS (Center for Nanoscience, München)
- 2007 Posterpreis der European Biophysical Society in London
- Seit 2007 Mitglied im "International Doctorate Program NanoBioTechnology"
Patente
- "Anordnung zum Untersuchen und/oder Manipulieren von Proben" DE 10 2007 010 890.0
- "Method for Data analysis", unter Begutachtung vom Europäischen Patentamt
Publikationen
- E.M. Puchner and H.E. Gaub. 2009. Exploring the conformation regulated function of titin kinase by mechanical single-molecule pump and probe. Angewandte Chemie Int. Ed. accepted
- Cordes T, Strackharn M, Stahl SW, Summerer W, Steinhauer C, Forthmann C, Puchner EM, Vogelsang J, Gaub HE, Tinnefeld P. 2009. Resolving Single-Molecule Assembled Patterns with Superresolution Blink-Microscopy. Nano Lett, 2009
- Stahl S.W., E.M. Puchner and H.E. Gaub. 2009. Photothermal cantilever actuation for fast single molecule force spectroscopy. Rev.Sci.Inst. 80(7).
- E.M. Puchner and H.E. Gaub. 2009. Force and function: Probing proteins with AFM-based force spectroscopy.. Curr.Opin.Struc.Biol19(5):605-14.
- Gumpp H., S.W. Stahl, M. Strackharn, E.M. Puchner and H.E. Gaub 2009. Ultrastable combined atomic force and total internal fluorescence microscope. Rev.Sci.Inst. 80(6).
- Gumpp H., E.M. Puchner, J. Zimmermann, U. Gerland, H.E. Gaub and K. Blank 2009. Triggering Enzymatic Activity with Force. Nano Lett, 9(9):3290-5.
- E.M. Puchner and Hermann E. Gaub Mechanoenzymatics and Nanoassembly of single molecules.Proceedings of the Nobel Symposium 138: Single Molecule Spectroscopy in Chemistry, Physics and Biology. Springer Series in Chemical Physics, Vol 96. ISBN: 978-3-642-02596-9
- E.M. Puchner, A. Alexandrovich, A.L. Kho, U. Hensen, L.V. Schafer, B. Brandmeier, F. Grater, H. Grubmuller, H.E. Gaub, and M. Gautel. 2008. Mechanoenzymatics of titin kinase. PNAS 105(36):13385-13390.
- Morfill, J., J. Neumann, K. Blank, U. Steinbach, E.M. Puchner, K.E. Gottschalk, and H.E. Gaub. 2008.Force-based analysis of multidimensional energy landscapes: application of dynamic force spectroscopy and steered molecular dynamics simulations to an antibody fragment-peptide complex.J Mol Biol 381(5):1253-1266.
- E.M. Puchner, G. Franzen, M. Gautel, and H.E. Gaub. 2008. Comparing proteins by their unfolding pattern. Biophys J 95(1):426-434.
- Kufer, S.K., E.M. Puchner, H. Gumpp, T. Liedl, and H.E. Gaub. 2008. Single-molecule cut-and-paste surface assembly. Science 319(5863):594-596.
- Kufer, S.K., M. Strackharn, S.W. Stahl, H. Gumpp, E.M. Puchner, and H.E. Gaub. 2008. Optically monitoring the mechanical assembly of single molecules. Nature Nanotechnology, 4(1):45-9
- E.M. Puchner, S.K. Kufer, M. Strackharn, S.W. Stahl, and H.E. Gaub. 2008. Nanoparticle Self-Assembly on a DNA-Scaffold Written by Single-Molecule Cut-and-Paste. Nano Letters 8(11):3692-3695